Actomyosin contractility and microtubules drive apical constriction in Xenopus bottle cells
نویسندگان
چکیده
منابع مشابه
Actomyosin Transports Microtubules and Microtubules Control Actomyosin Recruitment during Xenopus Oocyte Wound Healing
BACKGROUND Interactions between microtubules and actin filaments (F-actin) are critical for cellular motility processes ranging from directed cell locomotion to cytokinesis. However, the cellular bases of these interactions remain poorly understood. We have analyzed the role of microtubules in generation of a contractile array comprised of F-actin and myosin-2 that forms around wounds made in X...
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Several cell motility processes including cytokinesis and cell locomotion are dependent on the interplay of the microtubule and actomyosin cytoskeletons. However, because such processes are essentially visual phenomena, interactions between the two cytoskeletal systems have been difficult to study quantitatively. To overcome this difficulty, we have developed the Xenopus oocyte as an inducible,...
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Apical constriction typically accompanies inward folding of an epithelial sheet. In recent years there has been progress in understanding mechanisms of apical constriction and their contribution to morphogenetic processes. Sea urchin embryos form a specialized region of ectoderm, the ciliary band, which is a strip of epithelium, three to five cells wide, encircling the oral ectoderm and functio...
متن کاملEndocytosis Is Required for Efficient Apical Constriction during Xenopus Gastrulation
Coordinated apical constriction (AC) in epithelial sheets drives tissue invagination [1, 2] and is required for diverse morphogenetic movements such as gastrulation [3], neurulation [4, 5], and organogenesis [6]. We showed previously that actomyosin contractility drives AC in Xenopus laevis bottle cells [7]; however, it remained unclear whether it does so in concert with other processes. Here w...
متن کاملActin Transports Microtubules and Microtubules Control Actomyosin Recruitment during Xenopus Oocyte Wound Healing
Oocyte Preparation, Microinjection, and Wounding for 12–20 hr, and then incubated them for 12–20 hr in rhodamineantimouse (ICN, Aurora, OH), washed them for another for 12–20 Full-grown oocytes were obtained from adult Xenopus laevis and stripped of their follicle cells as previously described [S1]. Oocytes hr, and then viewed them. For detection of the Arp2/3 complex, rabbit anti-p16 antibodie...
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ژورنال
عنوان ژورنال: Developmental Biology
سال: 2007
ISSN: 0012-1606
DOI: 10.1016/j.ydbio.2007.08.010